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Biochemical Techniques- Flylab

View attached explanation and answer. Let me know if you have any questions.

Mahbubeh Arezomandi
BIBC 103
Fly Lab Report
You will present the data from your fly EMBA and ADH activity assays, and briefly
explain your findings and observations. The components that you will need to include in
your report is:
1. Data table (5 points): Refer to the “Template” tab of the “Fly lab data” sheet. Using the class
data fill out this table and include in your report. This data can be used to generate the
graphs below.
Strain
1118 (Female)
1118 (Male)
3976 (Female)
3976 (Male)
4062 (Female)
4062 (Male)

ST50 (avg.)
ST50 (stdev.) ST100 (avg.) ST100 (stdev.) Specific activity (U/mg) (avg.) Specific activity (U/mg) (stdev.)
17.875
1.652019
23
3.559026
30.04917737
12.87901414
19.75
2.061553
30.25
3.304038
27.76463098
10.77023107
14
3.162278
18.5
4.358899
0
0
14.4375
0.921389
18.75
1.258306
0
0
16.875
5.022864
24
7.302967
23.24434033
7.477978129
13.275
2.892951
22
7.702813
81.21422104
80.90238559

2. ST50 graph (10 points): Plot a female-male paired bar graph for three different genotypes
(1118, 3976, 4062) including the error bars with standard deviations. Perform 2-way
ANOVA on your samples with alpha 0.05 (default). Interpret your results based on the Pvalues. Are there any differences between samples (between different gender or genotype)?

Str…

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aalan

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